This comprehensive guide to the enzyme-linked immunospot (Elispot) assay offers clear, logical, and practical explanations for making informed choices to achieve optimal results. Whether you are a newcomer eager to learn or an experienced user seeking to refine your techniques, this book is packed with valuable insights and best practices derived from over twenty years of first-hand experience in the field.
Inside, you will discover:
- Step-by-step protocols: Detailed guidance on specific protocol steps, reagents, and materials.
- Enhanced understanding: Clear explanations that help newcomers grasp the essential elements of the Elispot technique.
- Best practices: Proven strategies that even seasoned Elispot users can apply to improve their assay results.
- Real-world applications: Insights drawn from the experiences of numerous colleagues and collaborators in various research fields.
The Elispot assay is a powerful tool for monitoring cellular responses at the single-cell level, enabling the detection of analytes such as cytokines, chemokines, and immunoglobulins (antibodies) in response to specific stimuli.
Authors:
Sylvia Janetzki (Author)
Edition:
1st ed. 2016
Publication Date:
November 10, 2016
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